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p p65  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology p p65
    P P65, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 809 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+nf%CE%BAb+p65/p-NF%CE%BAB+p65+Antibody/pm42830147-100-10-15
    Average 96 stars, based on 809 article reviews
    p p65 - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Astaxanthin alleviates altered hepatic lipid metabolism and oxidative stress in animals fed a high-sucrose diet
    Article Snippet: The sections were mounted on 3-aminopropyltriethoxysilane (Sigma-Aldrich, Buenos Aires, Argentina)-coated slides and a subsequent microwave pretreatment for antigen retrieval was performed. .. The samples were incubated in a humid chamber first with a specific primary antibody for Nrf2 (mouse monoclonal antibody; sc-365949; Santa Cruz Biotechnology) and p-NFκB p65 (mouse monoclonal antibody; sc-136548; Santa Cruz Biotechnology) (for 14–16 h at 4 °C) and then with biotin-conjugated secondary antibody (antimouse, 1:100 dilution, Sigma) for 30 min at room temperature. .. The reactions were developed using the streptavidin–biotin peroxidase method and diaminobenzidine (Sigma) as a chromogenic substrate.

    Article Title: Viniferin-Rich Phytocomplex from Vitis vinifera L. Plant Cell Culture Mitigates Neuroinflammation in BV2 Microglia Cells
    Article Snippet: Protein samples (20 μg) were separated on 10% SDS-PAGE [ ] and then blotted onto Midi Nitrocellulose membranes using a Trans-Blot Turbo Transfer Starter System (Bio-Rad Laboratories, Milan, Italy). .. Blots were incubated overnight at 4 °C with primary antibodies against pERK1/2 (1:1000, Cell Signaling Technology, Danvers, MA, USA), SIRT1 (1:1000, Santa Cruz Biotechnology, Dallas, TX, USA), NFκB p65 (1:1000, Santa Cruz Biotechnology), and p-NFκB p65 (1:500, Santa Cruz Biotechnology). .. After being washed with PBS containing 0.1% Tween, the nitrocellulose membranes were incubated with goat anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:3000, Jackson ImmunoResearch Labs, West Grove, PA, USA) for 2 h at room temperature (RT; 20–22 °C).

    Article Title: Omega-3 Polyunsaturated Fatty Acids Protect Neurological Function After Traumatic Brain Injury by Suppressing Microglial Transformation to the Proinflammatory Phenotype and Activating Exosomal NGF/TrkA Signaling.
    Article Snippet: Protein concentrations in supernatant were determined using a BCA protein assay kit (Abcam, Cambridge, UK), then equal amounts per gel lane (30 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes. .. The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated with primary antibodies targeting CD16+ (1:500; Santa Cruz), ADAM17 (1:500; Abcam), and TNF-α (1:500; Santa Cruz), TNFR1 (1:500; Santa Cruz), p-IκB (1:500; Santa Cruz), NF-κB p65 (1:500; Santa Cruz), p-NFκB p65(1:500; Santa Cruz), caspase-3 (1:3000; Abcam), Bax (1:1,000; Abcam), Bcl2 (1:2000; Abcam), NGF (1:1000, Abcam), TrkA (1:1,000; Abcam), and p-TrkA (1:1,000; Abcam). .. After incubation with an appropriate secondary antibody, immunolabeling was visualized using the Millipore ECL Western Blotting Detection System (Millipore, Billerica, MA, USA).

    Cell-Signaling:

    Article Title: Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis mice
    Article Snippet: .. Primary antibody iNOS (ab204017) was purchased from Abcam (Hong Kong) Limited, Hong Kong SAR, China; COX-2 (12282S), Nrf-2 (12721S), and NFκB p65 (8242S) were purchased from Cell Signaling Technology, Danvers, Massachusetts, U.S.A; mouse anti-ZO-1(339100) and loading control (MA515738D680) were purchased from ThermoFisher Scientific, Waltham, Massachusetts, USA; p-NFκB p65 (sc-166748), IκB- α (sc-1643), p-IκB- α (sc-8404), and secondary antibodies mouse anti-rabbit IgG-HRP (sc-2357) and m-IgGκ BP-HRP (sc-516102) were purchased from Santa Cruz Biotechnology, California, U.S.A. Dry A. auricula-judae mushroom was collected from the College of Horticulture, Jilin Agricultural University, Changchun 130118, China. ..

    Article Title: Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis mice
    Article Snippet: .. Primary antibody iNOS (ab204017) was purchased from Abcam (Hong Kong) Limited, Hong Kong SAR, China; COX-2 (12282S), Nrf-2 (12721S), and NFκB p65 (8242S) were purchased from Cell Signaling Technology, Danvers, Massachusetts, U.S.A; mouse anti-ZO-1(339100) and loading control (MA515738D680) were purchased from ThermoFisher Scientific, Waltham, Massachusetts, USA; p-NFκB p65 (sc-166748), IκB-α (sc1643), p-IκB-α (sc-8404), and secondary antibodies mouse anti-rabbit IgG-HRP (sc-2357) and m-IgGκ BP-HRP (sc-516102) were purchased from Santa Cruz Biotechnology, California, U.S.A. Dry A. auricula-judae mushroom was collected from the College of Horticulture, Jilin Agricultural University, Changchun 130118, China. ..

    Control:

    Article Title: Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis mice
    Article Snippet: .. Primary antibody iNOS (ab204017) was purchased from Abcam (Hong Kong) Limited, Hong Kong SAR, China; COX-2 (12282S), Nrf-2 (12721S), and NFκB p65 (8242S) were purchased from Cell Signaling Technology, Danvers, Massachusetts, U.S.A; mouse anti-ZO-1(339100) and loading control (MA515738D680) were purchased from ThermoFisher Scientific, Waltham, Massachusetts, USA; p-NFκB p65 (sc-166748), IκB- α (sc-1643), p-IκB- α (sc-8404), and secondary antibodies mouse anti-rabbit IgG-HRP (sc-2357) and m-IgGκ BP-HRP (sc-516102) were purchased from Santa Cruz Biotechnology, California, U.S.A. Dry A. auricula-judae mushroom was collected from the College of Horticulture, Jilin Agricultural University, Changchun 130118, China. ..

    Article Title: Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis mice
    Article Snippet: .. Primary antibody iNOS (ab204017) was purchased from Abcam (Hong Kong) Limited, Hong Kong SAR, China; COX-2 (12282S), Nrf-2 (12721S), and NFκB p65 (8242S) were purchased from Cell Signaling Technology, Danvers, Massachusetts, U.S.A; mouse anti-ZO-1(339100) and loading control (MA515738D680) were purchased from ThermoFisher Scientific, Waltham, Massachusetts, USA; p-NFκB p65 (sc-166748), IκB-α (sc1643), p-IκB-α (sc-8404), and secondary antibodies mouse anti-rabbit IgG-HRP (sc-2357) and m-IgGκ BP-HRP (sc-516102) were purchased from Santa Cruz Biotechnology, California, U.S.A. Dry A. auricula-judae mushroom was collected from the College of Horticulture, Jilin Agricultural University, Changchun 130118, China. ..



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